Most cosmetics contain water, carbon sources, and nutrients, which create favorable conditions for the growth and reproduction of microorganisms at appropriate temperature and pH. If the microbial content of cosmetics does not meet quality inspection standards, it can lead to product quality problems such as discoloration, bloating, sour odor, emulsion breakdown or layering. Strict microbial testing of cosmetics is the basic guarantee for ensuring their quality.
Like the microbiological testing of food products, the microbiology testing of cosmetics is also very important, which is an important aspect of the quality control of cosmetics, mainly aiming at microorganisms. By testing microorganism categories and indicators, comparing the results with corresponding standards, and determining whether microorganisms in cosmetics fall within the specified range of indicators. The main testing contents include the total number of bacteria and checking for different pathogenic bacteria. The range of bacteria contained by pathogenic bacteria in microbiology is relatively wide. The methods used for testing different types of microorganisms and pathogenic bacteria also vary. The specific testing methods refer to the "Guidelines for Cosmetic Microbial Testing" published by the country's hygiene standards. Relevant regulations clearly require that only specific categories of pathogenic bacteria need to be tested in cosmetic microbiology testing, narrowing the scope of testing.
In actual cosmetic microbiology testing, the sampling method used is mostly random sampling. Although this method represents a certain degree of accuracy in testing results, as the demand for cosmetic types and categories becomes increasingly diverse, traditional sampling methods are becoming more and more unable to meet higher requirements for quality inspection of cosmetics. If the representative of the samples is poor, the microbial testing results will not reflect the true situation of the quality of cosmetics. In addition, in the process of cosmetic microbiology testing, there are also factors such as systematic and accidental errors that can easily lead to large discrepancies between microbial testing results and actual results. This also indicates that cosmetic quality inspection institutions and personnel must continue to explore and optimize testing methods to improve the stability and accuracy of testing results.
In the collection of cosmetic samples, the rationality of the collection method must be ensured to make the sample collection representative. Usually, random sampling is carried out according to the number of each batch of cosmetics. The number of random samples should be at least two packaging units, and the amount of samples taken should not be less than 10 grams or 10 milliliters. The extracted samples must be numbered and registered, and inspection should be arranged as soon as possible. If inspection cannot be carried out immediately, avoid refrigeration or freezing and keep samples dry and cool at room temperature for preparation. In this process, contamination should be avoided.
In the bacterial count of cosmetic samples, the method of observing with the naked eye and magnifying glass is used to record the bacterial count in the culture dish, and the average bacterial count in the culture dish is calculated under the same dilution conditions. If the bacterial colonies have not grown over the entire culture dish, half of the culture dish lacks colonies while the other half has evenly distributed colonies. In this case, the number of colonies on the evenly distributed half can be counted and multiplied by two to obtain the total bacterial count of the entire culture dish. The appearance of flower-like or connecting bacterial colonies is regarded as not meeting the technical conditions.
Microbial contamination of cosmetics not only affects the quality of the product itself, but also seriously endangers the health and safety of consumers. Therefore, it is necessary to conscientiously implement national cosmetic quality production regulations, strengthen cosmetic microbiology testing, ensure that microorganisms in cosmetics meet relevant national regulations and standards, and prevent microbial contamination of cosmetics, thereby enhancing their quality and safety.
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